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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was discovered to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are described. Two supplemental splice variants ended up reported but in-depth nucleotide sequence details is not still accessible.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide usually contains the epitope acknowledged because of the antibody.
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This manual outlines further probable optimization of mobile and immunoassay parameters to make certain the best possible success are...
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to guarantee the absolute best outcomes are...
Antibodies sure to the blocking peptide no more bind to the epitope to the goal protein. By evaluating the staining from your blocked antibody versus the antibody alone, one can see which staining is unique.
Target Facts Cyclin E here belongs towards the remarkably conserved cyclin family, whose members are characterised by a dramatic periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins show unique expression and degradation designs which contribute for the temporal coordination of every mitotic function. Cyclin E types a posh with and functions like a regulatory subunit of CDK2, whose activity is required for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is also degraded as cells development by S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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